Three sweet orange cultivars at full mature stage with similar soluble solid, acidity, pH and ripening index were collected from an orange farm in Spain.

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Materials and Methods

Three sweet orange cultivars at full mature stage with similar soluble solid, acidity, pH and ripening index were collected from an orange farm in Spain. 10 sets of samples (5 oranges in each set) from each type of the orange were peeled, homogenised, then filtered (using 2 mm steel sieve) to produce fresh smooth orange juice samples. Vitamin C (ascorbic acid) were extracted from the unprocessed samples and analysed using HPLC method. The results were recorded as mg/100ml.

Additional 20 sets of Cultivar A samples were juiced using same method described above, and further processed using two processing methods. Half of the sample was processed via thermal pasteurisation at 80 °C for 30s. The other half was treated using HPP (high pressure process) at 200 MPa/25 °C for 1 min. Vitamin C (ascorbic acid) were extracted from the processed samples, and analysed using same method. 

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