Assess the viability of cells during conditions of elevated reactive oxygen species on proteasome impairment in the presence and absence of antioxidants.

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Proteasome impairment in neural cells

500-800 words

Paper format (Toxicology)

A literature review has four main objectives:

• It surveys the literature in your chosen area of study.
• It synthesises the information in that literature into a summary.
• It critically analyses the information gathered by identifying gaps in current knowledge; by showing limitations of theories and points of view; and by formulating areas for further research and reviewing areas of controversy.
• It presents the literature in an organised way.

Proteasome impairment in neural cells

Cells: Mouse Neural cells (e.g., N2a neuroblastoma/ Rat C6 Glioma cell line)

Compounds: AAPH (lipid peroxidation), H2O2 (oxidative stress), Rotenone (mitochondrial inhibitor), MG132 (proteasome inhibitor), Antioxidants (NAC, Sulforaphane, Vit E).

Techniques: Tissue Culture, Viability Assessment (MTT reduction assay, Cell-Titre Blue, Trypan Blue,), Proteasome activity assays, Reactive oxygen species assay, Antioxidant levels.

• Assess the viability of cells during conditions of elevated reactive oxygen species on proteasome impairment in the presence and absence of antioxidants.
• Determine whether proteasome activity is affected by mitochondrial impairment / lipid peroxidation. Does proteasome impairment exacerbate these situations?
• Can increased antioxidant levels protect cell viability and maintain proteasome activity.
• Does ROS target specific components of the Proteasome.
• Bioinformatics analysis of rotenone in neuronal cell models – Proteasome related changes in gene expression.